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You can multiply together all of the dilution factors to get total dilution factor.So here s the general rule: in order to be valid, the plate that you scale up should contain between 25 and 250 CFUs.Below are a few unusual situations things do go wrong, of course, and you should be able to recognize that.Great, it didn t work the first time, so now we re going to do it again.Scaling up means starting from a sample and figuring out how many were in the original brew, or stock, or whatever was originally there.Automatic downloads of regularly updated Virus Database.Use the scratchpad to check your plans, and if you get completely lost, click the.1 mL stock mL water Can you do it in your sleep.The trickier part is figuring out a series of dilutions that will achieve your overall dilution.Back to the lab Here are some problems, ranging from easy to a bit hard I did quite box of tricks 1.8 serial a series of dilutions with an overall dilution factor of 1/20,000, and then plated a grew a 1mL sample.Said the second sister enthusiastically.
Take 1 mL of that and put it in 49 mLs of water.
Overall dilution factor.1.1.01.0001 Multiply by the inverse I think I have the answer: 48 1/0.0001 480,000 CFUs per mL I did a series of dilutions as follows: 1 mL added to 9 mLs water 1 mL added to 99 mLs water.Avira Free antivirus eliminates many forms of malware, including worms, rootkits and costly dialers.The son was smart, charming, and funny, but not one of the princesses that he brought home was good enough for his mother.AVG Free Antivirus 2012 offers high quality basic protection for PCs.Sadly, the prince s mother was not blind or totally stupid.Based on me eating - I mean, counting - a single fun-size pack.
Put one mL of a stock into 99 mLs of water.
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